PCR detection kit for Enterobacteriaceae

The iQ-Check Enterobacteriaceae PCR Detection Kit by Bio-Rad is intended for the detection of Enterobacteriaceae in infant formula and related raw ingredients as well as environmental samples.

Due to the sensitivity and specificity of PCR, results can be obtained in as little as 3 h following a single enrichment in non-selective buffered peptone water (3564684 and 3554179).

The kit uses an optimised system of primers and probes to ensure high specificity. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result (negative result validation).

Developed as an open and flexible system, the kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs.

The kit includes: lysis reagent for DNA extraction, fluorescent probes, PCR amplification mix and positive and negative PCR controls.