Finding E. coli earlier and more accurately

US researchers have developed a new method to detect Shiga toxin-producing E. coli O157:H7 earlier and more accurately.

As part of a study into preharvest food safety in beef cattle, Kansas State University master’s student Lance Noll has developed and validated a molecular assay that can detect and quantify major genes specific to E. coli O157.

“Developing a method to detect E. coli before it can potentially contaminate the food supply benefits the beef industry by preventing costly recalls but also benefits the consumer by ensuring the safety of the beef supply,” Noll said.

The newly developed test is a molecular assay, or polymerase chain reaction, that detects bacteria based on genetic sequences, which are the bacteria’s ‘fingerprints’, according to TG Nagaraja, university distinguished professor of diagnostic medicine and pathobiology. The test is rapid and reportedly less labour-intensive than existing detection methods. The method can be automated and test many samples in a short period of time.

The test can be used in a diagnostic or research laboratory to accurately detect E. coli and can help with quality control in cattle facilities.

“The novelty of this test is that it targets four genes. We are constantly working on finding better and more sensitive ways to detect these pathogens of E. coli in cattle faeces,” Nagaraja said.

A US Department of Agriculture Coordinated Agriculture Project grant funded the research.