BMG Labtech PHERAstar FS microplate reader for antioxidant assay

Everyday metabolism, as well as stress and environmental pollutants, causes cells in the human body to produce molecules that are collectively known as reactive oxygen species (ROS). ROS can be either free radicals or can form free radicals through their interaction with biological molecules (ie, proteins, DNA/RNA and lipids). Too many free radicals are thought to play a role in cancer, ageing and other degenerative diseases, such as cardiovascular, Alzheimer’s and Parkinson’s.

A method was needed that could measure the antioxidant capacity of a substance, either directly from the food or indirectly in the blood after being consumed. The oxygen radical absorbance capacity (ORAC) assay is such a method, and can be conducted using the PHERAstar FS microplate reader from BMG Labtech. It is suitable for food manufacturers wishing to market their products as high in antioxidants.